{"title":"Hygia Reagents","description":"\u003cp\u003eShop Hygia Reagents in Canada at VitaminNorth. Every price includes shipping, orders ship in 2–4 business days, and unopened items can be returned within 30 days.\u003c\/p\u003e","products":[{"product_id":"hygia-reagents-dmem-cell-culture-medium-low-glucose-500-ml","title":"Hygia Reagents DMEM Cell Culture Medium Low Glucose - 500 mL","description":"\u003cp\u003eHygia Reagents DMEM (Dulbecco's Modified Eagle Medium) is a basal cell culture medium formulated to support the growth of many mammalian cell types. This low-glucose variant contains four times the amino acids and vitamins of the original Eagle's Minimal Essential Medium, making it suitable for diverse cell culture applications.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eContains 4× amino acids and vitamins compared to original Eagle's Medium\u003c\/li\u003e\n\u003cli\u003eSuitable for primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells\u003c\/li\u003e\n\u003cli\u003eIncludes phenol red pH indicator and sodium bicarbonate buffer system\u003c\/li\u003e\n\u003cli\u003eManufactured at cGMP-compliant facility with 0.1 μm sterile filtration\u003c\/li\u003e\n\u003cli\u003eEach lot undergoes sterility, pH, osmolality, endotoxin and cell culture testing\u003c\/li\u003e\n\u003cli\u003eRequires supplementation with Fetal Bovine Serum and 5–10% CO₂ environment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\u003cp\u003eRequires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Requires a 5–10% CO₂ environment to maintain physiological pH.\u003c\/p\u003e\n\u003ch2\u003eWho Is It For\u003c\/h2\u003e\u003cp\u003eThis product is designed for research laboratories and cell culture facilities working with mammalian cells, including primary cell types and established cell lines.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eBrand\u003c\/th\u003e\n\u003ctd\u003eHygia Reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\u003ch3\u003eWhat cell types is this DMEM medium suitable for?\u003c\/h3\u003e\u003cp\u003eDMEM is suitable for primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12.\u003c\/p\u003e\u003ch3\u003eWhat does this medium contain and not contain?\u003c\/h3\u003e\u003cp\u003eDMEM contains amino acids, vitamins, phenol red pH indicator, and a sodium bicarbonate buffer system. It contains no proteins, lipids, or growth factors.\u003c\/p\u003e\u003ch3\u003eWhat supplementation and conditions does DMEM require?\u003c\/h3\u003e\u003cp\u003eDMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS), and a 5–10% CO₂ environment to maintain physiological pH.\u003c\/p\u003e\u003ch3\u003eWhat quality control testing is performed on this product?\u003c\/h3\u003e\u003cp\u003eEach lot undergoes sterility testing, pH analysis, osmolality measurement, endotoxin testing, and cell culture testing. The product is manufactured at a cGMP-compliant facility with 0.1 μm sterile filtration.\u003c\/p\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What cell types is this DMEM medium suitable for?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"DMEM is suitable for primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12.\"}}, {\"@type\": \"Question\", \"name\": \"What does this medium contain and not contain?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"DMEM contains amino acids, vitamins, phenol red pH indicator, and a sodium bicarbonate buffer system. 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